Gene engineering of the adenovirus vector
نویسندگان
چکیده
منابع مشابه
Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function.
With the enhanced capacity of bioinformatics to interrogate extensive banks of sequence data, more efficient technologies are needed to test gene function predictions. Replication-deficient recombinant adenovirus (Ad) vectors are widely used in expression analysis since they provide for extremely efficient expression of transgenes in a wide range of cell types. To facilitate rapid, high-through...
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Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a reco...
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Adenovirus vectors (Ad) have been frequently used for cancer gene therapy research because of their high gene transduction efficiency. However, systemic administration of conventional Ad can lead to the acute accumulation of virus particles and transgene expression in the liver, which may cause severe hepatotoxicity. For these reasons, clinical application of Ad for systemic administration has ...
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BACKGROUND Hemophilia B, an X-linked disorder, is ideally suited for gene therapy. We investigated the use of a new gene therapy in patients with the disorder. METHODS We infused a single dose of a serotype-8-pseudotyped, self-complementary adenovirus-associated virus (AAV) vector expressing a codon-optimized human factor IX (FIX) transgene (scAAV2/8-LP1-hFIXco) in a peripheral vein in six pa...
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NORPA-PLCb4 GENE EXPRESSION IN THE RAT BRAIN AND RETINA P. Roustsa*. M. Abitbol., F. Ribeaudeau*, C. M&nil, M. Gerard’, M. Vekemans”, J. Mall& and J.-L. Dufier*. WERTO, Faculle de medecim NeckeEafmteMnl&s, Univwsitl Ren6 Oesmtes,~i&m &age, 156 rue & Vaugimd, 75015 Paris. “Laboraoire de Oenetique Mokulaire d” D&elqpemat Hum&, Faculte d: dd&m ~eckm-Enfam-MaIdea UniversW Reti Descartes, 5ihe &age,...
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ژورنال
عنوان ژورنال: Uirusu
سال: 2007
ISSN: 0042-6857
DOI: 10.2222/jsv.57.37